Lactogenesis in the rat. Metabolism of uridine diphosphate galactose by mammary gland

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Generalised uridine diphosphate galactose-4-epimerase deficiency.

The generalised form of epimerase deficiency galactosaemia has been described in only two children from unrelated families. Their progress is reported and three other affected children from these families are described. The initial presentation was similar to classic galactosaemia. Despite treatment all have shown poor growth and moderate learning difficulties. Three have sensorineural deafness...

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The Intermediate in the Uridine Diphosphate Galactose P-Epimerase Reaction

Incubation of UDP-galactose with substrate amounts of UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli for different times between 2 and 120 min, followed by reduction with NaB3H4 invariably yields a mixture of labeled UDP-glucose and UDP-galactose. Chemical degradation of tritiated hexoses, isolated after NsB3H4 reduction of 2-min incubation mixtures, shows all of the tritium assoc...

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The Intermediate in the Uridine Diphosphate Galactose P-Epimerase Reaction

Incubation of UDP-galactose with substrate amounts of UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli for different times between 2 and 120 min, followed by reduction with NaB3H4 invariably yields a mixture of labeled UDP-glucose and UDP-galactose. Chemical degradation of tritiated hexoses, isolated after NsB3H4 reduction of 2-min incubation mixtures, shows all of the tritium assoc...

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Enzymes of Galactose Synthesis in Mammary Gland and Mammary Tumors of the Rat.

As part of studies now under way in our laboratory devoted to determining the degree of loss or retention of functional enzymatic activities in the transformation of normal to neoplastic cells, it was deemed desirable to study, both in the normal mammary gland and in mammary tumors, the levels of certain enzymes specifically involved in the production of lactose. This substance is formed, perha...

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Frog brain uridine diphosphate galactose-N-acetylgalactosaminyl-N-acetylneuraminylgalactosylglucosylceramide galactosyltransferase.

1. The enzyme that catalyses the transfer of galactose from UDP-galactose to N-acetylgalactosaminyl-(1-->4)-N-acetylneuraminyl-(2-->3)-galactosyl-(1-->4)-glucosylceramide (G(M2)) was found mainly in the heavy- and light-microsomal fractions of the adult frog brain. 2. The subcellular distribution of the enzyme, UDP-galactose-G(M2) galactosyltransferase, parallels that of gangliosides in adult f...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1968

ISSN: 0306-3283

DOI: 10.1042/bj1060743